Hyaluronan Binding Assay (HBA) versus Sperm Penetration Assay (SPA). Can HBA replace the use of SPA test in male partner screening before in vitro fertilization?

Jelena Lazarevic, Maria Wikarczuk, Stephen G Somkuti, Larry I Barmat, Jay S Schinfeld, Scott E Smith


Structured abstract:

Objective: To determine if a less expensive, easier, and faster to perform HBA test is clinically equal to the more complicated, technically challenging and expensive SPA test as a reliable indicator of sperm fertilizing capacity.

Design: Prospective study.

Setting: Andrology laboratory within In Vitro Fertilization Program.

Patient(s): Semen samples from 26 infertility couples were analyzed. Both, normal and male factor patients were included.

Intervention(s): Male partner screening with the HBA and the SPA tests.

Main Outcome Measure(s): Relationship between HBA and SPA test results.

Result(s): The data obtained in this study showed no statistically significant relationship between the HBA and SPA results. The mean HBA scores 76.3%, 61.3% and 76.8% were statistically not significantly different as compared to patients with negative (<5), grey zone (5-8) and for positive (>8) sperm capacitation index values.

Conclusion(s): The HBA is not predictive of the results of the SPA. Hence, if the SPA is a reliable indicator of the fertilizing capacity of human spermatozoa then the HBA is not and we do not suggest replacing SPA with HBA.

Key Words: Hyaluronan-binding assay, sperm penetration assay, male partner screening


Hyaluronan-binding assay, sperm penetration assay, male partner screening

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Journal of Experimental & Clinical Assisted Reproduction (JECAR)

Published by The Sims Institute Press Ltd Dublin, Los Angeles & New York 2008-2014